Training on RT-PCR, arranged by APHL and CDC…………………..

How should I say, what a training it was. It was both educational and recreational I guess!!!!

1st day at DBD international Molecular Training

Participant Country:

Bangladesh, Ghana, Senegal, Vietnam


At first, we introduce ourselves, then all other CDC and APHL person introduce themselves. Then we had a pre-test.


Pre-test: They said that this test is not for yourselves it for us. We want to know how much do you know about the basic things of RT PCR and about those bacteria we will deal. The pre-test was filled with ques of how to identify Spn Hi and Nmen, the precautionary technique of RT-PCR, and so many things. It was quite ok. For me listening so many things about RT PCR through Hasan vai and Roly apu in our lab meeting was helpful. Then they started lectures according to agenda.


Lecture 1:  Laboratory methods for detection of HI, SP, NM, General overview of methods for detection and characterization of 3 major bacterial meningitis pathogens

By Lesley McGee

The first lecture was given by Lesley, it was really a nice presentation. She gave an overall idea about HI, Spn, and Nmen detection. She also mentioned Bangladesh during one of her slide showing rapid detection method of SPN by Binax kit. She mentioned Spn sensitivity to optochin for its identification, as I did not know the real mechanism why Spn being sensitive to optochin, so I asked them also, why Spn is sensitive to optochin they said they also don’t know …

Lecture 2:  Principles and considerations of Real-time PCR Introduction to basic principles and chemistries; applications and methodology

Srinivasan Velusamy

He mentioned each and every basic thing to learn about RT PCR. In his session, Hasan vai did very good conversation with them. He praised Hasan vai for asking questions and getting a good point to ask a question.

I asked Sri to tell me more openly about RT PCR technique and threshold, as I am the person never had hands on RT PCR work. He said he will keep that in mind and help me to understand each and everything.

Lecture 3: DNA extraction methods Overview of extraction methods; considerations for bacterial species and specimen type

Lesley McGee

Lesley again gave a lecture on DNA extraction. She mentioned that doing Qiagen manual extraction is best for DNA extraction. She will give us a hands-on training tomorrow.

Stephanie Schwartz also talked with us and we got to know each other primarily. She mentioned that she came here at DSH and remember the routine lab where every sample comes, it seems very interesting to her.

After that we had lunch

Laboratory Orientation (L172 and 3 floor IDL lab space) Brief lab tour and BSL-3 orientation

After lunch we visited APHL lab, it is a nice lab, so many PCR and Extraction robots were everywhere. This lab works with Fungi, Virus, and Bacteria. So there is BSL-3 (biosafety level-3) lab. We visited the BSL-3 lab. In their lab, every PCR machine has different funny names under STAR Wars character (Star war is a movie name, I found it quite interesting)

Day 1 Wrap Up

Welcome Reception (DoubleTree Hotel): We come back to the hotel. Had dinner with everyone.